pGLO Lab

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB Carpet          White          White - pGLO LB/amp       0          White          White + pGLO LB/amp      75          White          White + pGLO LB/amp/ara     250          Green          Green

2.	What two new traits do your transformed bacteria have?
	Ability to glow and to be Ampicillin resistance
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	About 1,000, because we added 25 uL ro each plate and one plate got 250 bacteria colonies, since 25 times 10 is 250 you do 100 times 10 and you get 1,000 bactera.
4.	What is the role of arabinose in the plates?
	To give the sugar gene so it can glow
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	1. It can be used to study jellyfish, like how they look or genes that you need to see 2. Can be used in fluorescence microscopy to able to see cells or whatever you want to see under the microscope easier 3.And can be used to see different genes in animals
6.	Give an example of another application of genetic engineering.

Another example is mass produce of vaccinations